molecular biology

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Curated by: Animated biology With arpan (99 videos)

Currently Playing: 5 prime end labelling | end labeling of DNA | How do you label the prime ends of the DNA molecule?

This video explains the concept behind 5prime end labelling and how that could be useful for labelling probes. These labelled probes can be used in the in situ hybridization or Southern blot.

Tracks in this Playlist

Chromosome structure | Chromatin organization | 3D chromatin | levels of organization in chromosomes
Euchromatin vs heterochromatin | Molecular differences between Euchromatin & heterochromatin| molbio
Satellite DNA | Minisatellite and Microsatellite | Short Tandem Repeats | Mol-bio
Centromeres | Centromeres and Kinetochores | Chromosomal organization | centromere function
Histone variants | Where are histone variants found? | Function of Histone variants | Molbio
Kinetochore | Structure and Function | Molecular biology
Prokaryotic Transcription overview
Transcription in eukaryotes overview
Eukaryotic gene expression regulation: concept of chromatin
Transcription in eukaryotes | Chromatin-centric view of transcription | RNA pol II transcripts
Enhancers | Transcriptional regulation by Enhancers | Enhancer promoter loop
The Lac Operon
trp operon
Chromatin structure : Nucleosome
Histone modifications (Introduction)
Nucleosome remodeling complex( introduction)
Poly Adenylation of eukaryotic mRNA
5 prime capping of eukaryotic mRNA
Transcription factors | general transcription factors | transcription factor networks | Molbio
DNA Binding motifs overview
Pseudogene | What is the function of pseudogene? | What is an example of a pseudogene in humans?
Untranslated regions : how 5' and 3' UTRs regulate transcription and translation | 3' and 5' UTR
Site specific recombination | How do site specific Recombinases work?
Retrotransposons | Retroviral retro transposon | Non LTR retrotransposon | LINEs and SINEs | molbio
Replication 101: Prokaryotic vs Eukaryotic replication
Replication : Eukaryotic vs prokaryotic replication Initiation | CSIR NET | GATE | IIT JAM | GATB
DNA Polymerase in Prokaryotes and their mechanism of action( DNA Pol I ,DNA Pol II and DNA Pol III)
DNA cloning (overview) | gene cloning | What is the purpose of DNA cloning? | genetic engineering
plasmid copy number regulation | What is meant by copy number of plasmid? | low copy number plasmid
Plasmids | Cloning vectors: Plasmids | Why do we use plasmids in RDT? | features of a plasmid
Bacterial transformation
Expression vectors | What is in an expression vector? | applications of expression vectors
2D gel electrophoresis
Tet on -Tet off system
cDNA library || How cDNA library is constructed? || What are DNA libraries used for?
Beer Lambert law: derivation and usage
Flow cytometry : basic principles | What the use of flow cytometry ? | Cell sorting by FACS
genomic DNA library
Yeast artificial chromosome (YAC) | What are the components of yeast artificial chromosome?
polymerase chain reaction ( PCR) | What are the 3 main steps in a PCR reaction?
5 prime end labelling | end labeling of DNA | How do you label the prime ends of the DNA molecule?
SWI/SNF Nucleosome remodeling complex
promoter elements : Regulation of gene expression
How antibiotics can interfere in Transcription?
Enzymes used in Recombinant DNA Technology
Translation in Prokaryotes (overview)
CRISPR Cas9 : How CRISPR can be performed in the lab ?
CRISPR-Cas9 : Introduction and discovery
Aminoacylation of tRNA: translation 101
splicing mechanism and its importance (overview) | How does splicing happen? | Molbio
splicing mechanism and its importance (overview) | How does splicing happen? | Molbio
Hot start PCR || principle and usage
Reverse Transcriptase PCR (RT PCR) | What are the applications of RT PCR ? | RT PCR protocol
Gradient PCR
Multiplex PCR
Inverse PCR
Touch down PCR
Gateway cloning | How does Gateway cloning work? | What are the advantages of Gateway cloning? |
restriction fragment length polymorphism (RFLP) |Principle | applications | limitations
southern blotting | southern hybridization | What is the principle of Southern blotting?
northern blotting technique | northern hybridization | What is the principle of Northern blotting?
Microarray Technique || DNA Microarray || Gene expression analysis using microarray
Fluorescence In Situ Hybridization (FISH) || Application of FISH || Clinical relevance of FISH
In-situ hybridization: Technique to detect mRNA localization || application of situ hybridization
Immunohistochemistry | How to perform immunohistochemistry? | application of immunohistochemistry
Immunofluorescence | Direct and Indirect Immunofluorescence | clinical application of IF.
Coombs Test || Antiglobulin Test || Direct Coombs test and indirect Coombs test
Transposons | Transposable elements | Types of transposons| how transposons work?
GCaMP | GCaMP calcium indicator | How GCaMP works? | GCaMP application in neuroscience
Nucleotide excision repair | NER | DNA repair mechanism animation| xeroderma pigmentosum| DNA repair
Reporter gene | Reporter genes and their importance | Reporter gene assay | What are reporter genes?
Topoisomerase 1 and 2 mechanism | How Topoisomerase works? | Animated biology | Molecular biology
Luciferase reporter assay | What is luciferase assay used for? | Applications of Luciferase assay
Metabotropic receptors | How do metabotropic receptors work? | Examples of metabotropic receptors
NHEJ | Non-homologous end joining | What proteins are involved in non-homologous end joining?
Linkers and Adaptors for Cloning | molbio in 3 minutes | cloning adaptors
MALDI-TOF MS | What are the applications of MALDI-TOF? | Mass spectrometry.
Differential Scanning Fluorimetry (DSF) | DSF application | Thermal stability of protein
Protein tagging | What is the purpose of tagging proteins? | Applications of protein tagging
fluorescence correlation spectroscopy | FCS | How does FCS work? | Biological applications of FCS
LINEs and SINEs | Poly A retrotransposon | Genetic organization of LINE and SINE | Mol bio
Core promoter elements | TATA box | BRE element | What is the importance of core promoter elements.
micro RNA | What is microRNA (miRNA)? | How miRNAs work? | How miRNAs are detected experimentally?
Epithelial to Mesenchymal Transition | EMT | Molecular mechanism of EMT | significance of EMT
Poly A retrotransposon | LINE and SINEs
DNA Gyrase
Shine Dalgarno and Kojak sequence | Molecular biology | Quick concepts playlist
Puromycin | Mechanism of action | Where does puromycin bind in the ribosome? | How puromycin works?
Enzymes in DNA replication | Eukaryotic vs Prokaryotic DNA polymerase
Telomere replication | The End replication problem | Aging and Telomere.
DNA replication in Eukaryotes | Prokaryotic vs Eukaryotic DNA replication | Molbio | CSIR NET
Genomic Imprinting | How genomic Imprinting works at molecular level ?
The ubiquitin-proteasome mediated protein degradation pathway | Ubiquitin ligase | Proteasome
Histone methylation | Histone modification | Gene expression regulation
DNA vs Histone methylation | How DNA and histone methylation regulate gene expression?
gene expression regulation by Cis regulatory elements | enhancer and promoter | how enhancers work?
Nucleosome Explained in 5 Minutes | Chromatin Structure Made Easy | Molecular Biology
Single-Cell Transcriptomics Explained | scRNA-seq Basics | Molecular Biology
Structure of chromosome | Nucleosome | Beads on a String | 30nm Fiber | chromatin packing | Mol bio