molecular biology

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Currently Playing: cDNA library || How cDNA library is constructed? || What are DNA libraries used for?

this video describes how cDNA libraries are created and about the screening process. This video would answer the following questions like : What is cDNA library used for? What is a cDNA library and what is its purpose? How cDNA library is constructed? What is cDNA and why is it important? What are the applications of cDNA library? What is a cDNA library and what is its purpose? What is the advantage of using a cDNA library? What are DNA libraries used for?

Tracks in this Playlist

Chromosome structure | Chromatin organization | 3D chromatin | levels of organization in chromosomes
Euchromatin vs heterochromatin | Molecular differences between Euchromatin & heterochromatin| molbio
Satellite DNA | Minisatellite and Microsatellite | Short Tandem Repeats | Mol-bio
Centromeres | Centromeres and Kinetochores | Chromosomal organization | centromere function
Histone variants | Where are histone variants found? | Function of Histone variants | Molbio
Kinetochore | Structure and Function | Molecular biology
Prokaryotic Transcription overview
Transcription in eukaryotes overview
Eukaryotic gene expression regulation: concept of chromatin
Transcription in eukaryotes | Chromatin-centric view of transcription | RNA pol II transcripts
Enhancers | Transcriptional regulation by Enhancers | Enhancer promoter loop
The Lac Operon
trp operon
Chromatin structure : Nucleosome
Histone modifications (Introduction)
Nucleosome remodeling complex( introduction)
Poly Adenylation of eukaryotic mRNA
5 prime capping of eukaryotic mRNA
Transcription factors | general transcription factors | transcription factor networks | Molbio
DNA Binding motifs overview
Pseudogene | What is the function of pseudogene? | What is an example of a pseudogene in humans?
Untranslated regions : how 5' and 3' UTRs regulate transcription and translation | 3' and 5' UTR
Site specific recombination | How do site specific Recombinases work?
Retrotransposons | Retroviral retro transposon | Non LTR retrotransposon | LINEs and SINEs | molbio
Replication 101: Prokaryotic vs Eukaryotic replication
Replication : Eukaryotic vs prokaryotic replication Initiation | CSIR NET | GATE | IIT JAM | GATB
DNA Polymerase in Prokaryotes and their mechanism of action( DNA Pol I ,DNA Pol II and DNA Pol III)
DNA cloning (overview) | gene cloning | What is the purpose of DNA cloning? | genetic engineering
plasmid copy number regulation | What is meant by copy number of plasmid? | low copy number plasmid
Plasmids | Cloning vectors: Plasmids | Why do we use plasmids in RDT? | features of a plasmid
Bacterial transformation
Expression vectors | What is in an expression vector? | applications of expression vectors
2D gel electrophoresis
Tet on -Tet off system
cDNA library || How cDNA library is constructed? || What are DNA libraries used for?
Beer Lambert law: derivation and usage
Flow cytometry : basic principles | What the use of flow cytometry ? | Cell sorting by FACS
genomic DNA library
Yeast artificial chromosome (YAC) | What are the components of yeast artificial chromosome?
polymerase chain reaction ( PCR) | What are the 3 main steps in a PCR reaction?
5 prime end labelling | end labeling of DNA | How do you label the prime ends of the DNA molecule?
SWI/SNF Nucleosome remodeling complex
promoter elements : Regulation of gene expression
How antibiotics can interfere in Transcription?
Enzymes used in Recombinant DNA Technology
Translation in Prokaryotes (overview)
CRISPR Cas9 : How CRISPR can be performed in the lab ?
CRISPR-Cas9 : Introduction and discovery
Aminoacylation of tRNA: translation 101
splicing mechanism and its importance (overview) | How does splicing happen? | Molbio
splicing mechanism and its importance (overview) | How does splicing happen? | Molbio
Hot start PCR || principle and usage
Reverse Transcriptase PCR (RT PCR) | What are the applications of RT PCR ? | RT PCR protocol
Gradient PCR
Multiplex PCR
Inverse PCR
Touch down PCR
Gateway cloning | How does Gateway cloning work? | What are the advantages of Gateway cloning? |
restriction fragment length polymorphism (RFLP) |Principle | applications | limitations
southern blotting | southern hybridization | What is the principle of Southern blotting?
northern blotting technique | northern hybridization | What is the principle of Northern blotting?
Microarray Technique || DNA Microarray || Gene expression analysis using microarray
Fluorescence In Situ Hybridization (FISH) || Application of FISH || Clinical relevance of FISH
In-situ hybridization: Technique to detect mRNA localization || application of situ hybridization
Immunohistochemistry | How to perform immunohistochemistry? | application of immunohistochemistry
Immunofluorescence | Direct and Indirect Immunofluorescence | clinical application of IF.
Coombs Test || Antiglobulin Test || Direct Coombs test and indirect Coombs test
Transposons | Transposable elements | Types of transposons| how transposons work?
GCaMP | GCaMP calcium indicator | How GCaMP works? | GCaMP application in neuroscience
Nucleotide excision repair | NER | DNA repair mechanism animation| xeroderma pigmentosum| DNA repair
Reporter gene | Reporter genes and their importance | Reporter gene assay | What are reporter genes?
Topoisomerase 1 and 2 mechanism | How Topoisomerase works? | Animated biology | Molecular biology
Luciferase reporter assay | What is luciferase assay used for? | Applications of Luciferase assay
Metabotropic receptors | How do metabotropic receptors work? | Examples of metabotropic receptors
NHEJ | Non-homologous end joining | What proteins are involved in non-homologous end joining?
Linkers and Adaptors for Cloning | molbio in 3 minutes | cloning adaptors
MALDI-TOF MS | What are the applications of MALDI-TOF? | Mass spectrometry.
Differential Scanning Fluorimetry (DSF) | DSF application | Thermal stability of protein
Protein tagging | What is the purpose of tagging proteins? | Applications of protein tagging
fluorescence correlation spectroscopy | FCS | How does FCS work? | Biological applications of FCS
LINEs and SINEs | Poly A retrotransposon | Genetic organization of LINE and SINE | Mol bio
Core promoter elements | TATA box | BRE element | What is the importance of core promoter elements.
micro RNA | What is microRNA (miRNA)? | How miRNAs work? | How miRNAs are detected experimentally?
Epithelial to Mesenchymal Transition | EMT | Molecular mechanism of EMT | significance of EMT
Poly A retrotransposon | LINE and SINEs
DNA Gyrase
Shine Dalgarno and Kojak sequence | Molecular biology | Quick concepts playlist
Puromycin | Mechanism of action | Where does puromycin bind in the ribosome? | How puromycin works?
Enzymes in DNA replication | Eukaryotic vs Prokaryotic DNA polymerase
Telomere replication | The End replication problem | Aging and Telomere.
DNA replication in Eukaryotes | Prokaryotic vs Eukaryotic DNA replication | Molbio | CSIR NET
Genomic Imprinting | How genomic Imprinting works at molecular level ?
The ubiquitin-proteasome mediated protein degradation pathway | Ubiquitin ligase | Proteasome
Histone methylation | Histone modification | Gene expression regulation
DNA vs Histone methylation | How DNA and histone methylation regulate gene expression?
gene expression regulation by Cis regulatory elements | enhancer and promoter | how enhancers work?
Nucleosome Explained in 5 Minutes | Chromatin Structure Made Easy | Molecular Biology
Single-Cell Transcriptomics Explained | scRNA-seq Basics | Molecular Biology
Structure of chromosome | Nucleosome | Beads on a String | 30nm Fiber | chromatin packing | Mol bio