molecular biology

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Curated by: Animated biology With arpan (99 videos)

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Currently Playing: genomic DNA library

this video describes the process of making and screening genomic libraries in details.

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Tracks in this Playlist

• Chromosome structure | Chromatin organization | 3D chromatin | levels of organization in chromosomes
• Euchromatin vs heterochromatin | Molecular differences between Euchromatin & heterochromatin| molbio
• Satellite DNA | Minisatellite and Microsatellite | Short Tandem Repeats | Mol-bio
• Centromeres | Centromeres and Kinetochores | Chromosomal organization | centromere function
• Histone variants | Where are histone variants found? | Function of Histone variants | Molbio
• Kinetochore | Structure and Function | Molecular biology
• Prokaryotic Transcription overview
• Transcription in eukaryotes overview
• Eukaryotic gene expression regulation: concept of chromatin
• Transcription in eukaryotes | Chromatin-centric view of transcription | RNA pol II transcripts
• Enhancers | Transcriptional regulation by Enhancers | Enhancer promoter loop
• The Lac Operon
• trp operon
• Chromatin structure : Nucleosome
• Histone modifications (Introduction)
• Nucleosome remodeling complex( introduction)
• Poly Adenylation of eukaryotic mRNA
• 5 prime capping of eukaryotic mRNA
• Transcription factors | general transcription factors | transcription factor networks | Molbio
• DNA Binding motifs overview
• Pseudogene | What is the function of pseudogene? | What is an example of a pseudogene in humans?
• Untranslated regions : how 5' and 3' UTRs regulate transcription and translation | 3' and 5' UTR
• Site specific recombination | How do site specific Recombinases work?
• Retrotransposons | Retroviral retro transposon | Non LTR retrotransposon | LINEs and SINEs | molbio
• Replication 101: Prokaryotic vs Eukaryotic replication
• Replication : Eukaryotic vs prokaryotic replication Initiation | CSIR NET | GATE | IIT JAM | GATB
• DNA Polymerase in Prokaryotes and their mechanism of action( DNA Pol I ,DNA Pol II and DNA Pol III)
• DNA cloning (overview) | gene cloning | What is the purpose of DNA cloning? | genetic engineering
• plasmid copy number regulation | What is meant by copy number of plasmid? | low copy number plasmid
• Plasmids | Cloning vectors: Plasmids | Why do we use plasmids in RDT? | features of a plasmid
• Bacterial transformation
• Expression vectors | What is in an expression vector? | applications of expression vectors
• 2D gel electrophoresis
• Tet on -Tet off system
• cDNA library || How cDNA library is constructed? || What are DNA libraries used for?
• Beer Lambert law: derivation and usage
• Flow cytometry : basic principles | What the use of flow cytometry ? | Cell sorting by FACS
• genomic DNA library
• Yeast artificial chromosome (YAC) | What are the components of yeast artificial chromosome?
• polymerase chain reaction ( PCR) | What are the 3 main steps in a PCR reaction?
• 5 prime end labelling | end labeling of DNA | How do you label the prime ends of the DNA molecule?
• SWI/SNF Nucleosome remodeling complex
• promoter elements : Regulation of gene expression
• How antibiotics can interfere in Transcription?
• Enzymes used in Recombinant DNA Technology
• Translation in Prokaryotes (overview)
• CRISPR Cas9 : How CRISPR can be performed in the lab ?
• CRISPR-Cas9 : Introduction and discovery
• Aminoacylation of tRNA: translation 101
• splicing mechanism and its importance (overview) | How does splicing happen? | Molbio
• splicing mechanism and its importance (overview) | How does splicing happen? | Molbio
• Hot start PCR || principle and usage
• Reverse Transcriptase PCR (RT PCR) | What are the applications of RT PCR ? | RT PCR protocol
• Gradient PCR
• Multiplex PCR
• Inverse PCR
• Touch down PCR
• Gateway cloning | How does Gateway cloning work? | What are the advantages of Gateway cloning? |
• restriction fragment length polymorphism (RFLP) |Principle | applications | limitations
• southern blotting | southern hybridization | What is the principle of Southern blotting?
• northern blotting technique | northern hybridization | What is the principle of Northern blotting?
• Microarray Technique || DNA Microarray || Gene expression analysis using microarray
• Fluorescence In Situ Hybridization (FISH) || Application of FISH || Clinical relevance of FISH
• In-situ hybridization: Technique to detect mRNA localization || application of situ hybridization
• Immunohistochemistry | How to perform immunohistochemistry? | application of immunohistochemistry
• Immunofluorescence | Direct and Indirect Immunofluorescence | clinical application of IF.
• Coombs Test || Antiglobulin Test || Direct Coombs test and indirect Coombs test
• Transposons | Transposable elements | Types of transposons| how transposons work?
• GCaMP | GCaMP calcium indicator | How GCaMP works? | GCaMP application in neuroscience
• Nucleotide excision repair | NER | DNA repair mechanism animation| xeroderma pigmentosum| DNA repair
• Reporter gene | Reporter genes and their importance | Reporter gene assay | What are reporter genes?
• Topoisomerase 1 and 2 mechanism | How Topoisomerase works? | Animated biology | Molecular biology
• Luciferase reporter assay | What is luciferase assay used for? | Applications of Luciferase assay
• Metabotropic receptors | How do metabotropic receptors work? | Examples of metabotropic receptors
• NHEJ | Non-homologous end joining | What proteins are involved in non-homologous end joining?
• Linkers and Adaptors for Cloning | molbio in 3 minutes | cloning adaptors
• MALDI-TOF MS | What are the applications of MALDI-TOF? | Mass spectrometry.
• Differential Scanning Fluorimetry (DSF) | DSF application | Thermal stability of protein
• Protein tagging | What is the purpose of tagging proteins? | Applications of protein tagging
• fluorescence correlation spectroscopy | FCS | How does FCS work? | Biological applications of FCS
• LINEs and SINEs | Poly A retrotransposon | Genetic organization of LINE and SINE | Mol bio
• Core promoter elements | TATA box | BRE element | What is the importance of core promoter elements.
• micro RNA | What is microRNA (miRNA)? | How miRNAs work? | How miRNAs are detected experimentally?
• Epithelial to Mesenchymal Transition | EMT | Molecular mechanism of EMT | significance of EMT
• Poly A retrotransposon | LINE and SINEs
• DNA Gyrase
• Shine Dalgarno and Kojak sequence | Molecular biology | Quick concepts playlist
• Puromycin | Mechanism of action | Where does puromycin bind in the ribosome? | How puromycin works?
• Enzymes in DNA replication | Eukaryotic vs Prokaryotic DNA polymerase
• Telomere replication | The End replication problem | Aging and Telomere.
• DNA replication in Eukaryotes | Prokaryotic vs Eukaryotic DNA replication | Molbio | CSIR NET
• Genomic Imprinting | How genomic Imprinting works at molecular level ?
• The ubiquitin-proteasome mediated protein degradation pathway | Ubiquitin ligase | Proteasome
• Histone methylation | Histone modification | Gene expression regulation
• DNA vs Histone methylation | How DNA and histone methylation regulate gene expression?
• gene expression regulation by Cis regulatory elements | enhancer and promoter | how enhancers work?
• Nucleosome Explained in 5 Minutes | Chromatin Structure Made Easy | Molecular Biology
• Single-Cell Transcriptomics Explained | scRNA-seq Basics | Molecular Biology
• Structure of chromosome | Nucleosome | Beads on a String | 30nm Fiber | chromatin packing | Mol bio