molecular biology

Currently Playing: southern blotting | southern hybridization | What is the principle of Southern blotting?

You must be wondering what is southern bloting ? ;What is the purpose of using Southern blotting? ;What is the difference between PCR and Southern blot?;What is Southern and Northern blotting? - all these questions would be answered in this video. Southern blotting is a laboratory technique used to detect a specific DNA sequence in a blood or tissue sample. A restriction enzyme is used to cut a sample of DNA into fragments that are separated using gel electrophoresis. The DNA fragments are transferred out of the gel to the surface of a membrane. The membrane is the hybridised with radiolabeled probes and the patterns are visualised using autoradiogram.